Rationale for developing new virus vectors to analyze gene function in grasses through virus-induced gene silencing.
Identifieur interne : 000450 ( Main/Exploration ); précédent : 000449; suivant : 000451Rationale for developing new virus vectors to analyze gene function in grasses through virus-induced gene silencing.
Auteurs : Hema Ramanna [États-Unis] ; Xin Shun Ding ; Richard S. NelsonSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2013.
Descripteurs français
- KwdFr :
- Annotation de séquence moléculaire (MeSH), Bromoviridae (génétique), Gènes de plante (MeSH), Interférence par ARN (MeSH), Poaceae (génétique), Poaceae (virologie), Régulation de l'expression des gènes végétaux (MeSH), Techniques de knock-down de gènes (MeSH), Transduction génétique (MeSH), Vecteurs génétiques (MeSH), Virus des mosaïques (génétique).
- MESH :
English descriptors
- KwdEn :
- Bromoviridae (genetics), Gene Expression Regulation, Plant (MeSH), Gene Knockdown Techniques (MeSH), Genes, Plant (MeSH), Genetic Vectors (MeSH), Molecular Sequence Annotation (MeSH), Mosaic Viruses (genetics), Poaceae (genetics), Poaceae (virology), RNA Interference (MeSH), Transduction, Genetic (MeSH).
- MESH :
Abstract
The exploding availability of genome and EST-based sequences from grasses requires a technology that allows rapid functional analysis of the multitude of genes that these resources provide. There are several techniques available to determine a gene's function. For gene knockdown studies, silencing through RNAi is a powerful tool. Gene silencing can be accomplished through stable transformation or transient expression of a fragment of a target gene sequence. Stable transformation in rice, maize, and a few other species, although routine, remains a relatively low-throughput process. Transformation in other grass species is difficult and labor-intensive. Therefore, transient gene silencing methods including Agrobacterium-mediated and virus-induced gene silencing (VIGS) have great potential for researchers studying gene function in grasses. VIGS in grasses already has been used to determine the function of genes during pathogen challenge and plant development. It also can be used in moderate-throughput reverse genetics screens to determine gene function. However, the number of viruses modified to serve as silencing vectors in grasses is limited, and the silencing phenotype induced by these vectors is not optimal: the phenotype being transient and with moderate penetration throughout the tissue. Here, we review the most recent information available for VIGS in grasses and summarize the strengths and weaknesses in current virus-grass host systems. We describe ways to improve current virus vectors and the potential of other grass-infecting viruses for VIGS studies. This work is necessary because VIGS for the foreseeable future remains a higher throughput and more rapid system to evaluate gene function than stable transformation.
DOI: 10.1007/978-1-62703-278-0_2
PubMed: 23386292
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Nelson</name></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="eISSN">1940-6029</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bromoviridae (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Gene Knockdown Techniques (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Genetic Vectors (MeSH)</term><term>Molecular Sequence Annotation (MeSH)</term><term>Mosaic Viruses (genetics)</term><term>Poaceae (genetics)</term><term>Poaceae (virology)</term><term>RNA Interference (MeSH)</term><term>Transduction, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Annotation de séquence moléculaire (MeSH)</term><term>Bromoviridae (génétique)</term><term>Gènes de plante (MeSH)</term><term>Interférence par ARN (MeSH)</term><term>Poaceae (génétique)</term><term>Poaceae (virologie)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Techniques de knock-down de gènes (MeSH)</term><term>Transduction génétique (MeSH)</term><term>Vecteurs génétiques (MeSH)</term><term>Virus des mosaïques (génétique)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bromoviridae</term><term>Mosaic Viruses</term><term>Poaceae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bromoviridae</term><term>Poaceae</term><term>Virus des mosaïques</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Poaceae</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Poaceae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Gene Knockdown Techniques</term><term>Genes, Plant</term><term>Genetic Vectors</term><term>Molecular Sequence Annotation</term><term>RNA Interference</term><term>Transduction, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Annotation de séquence moléculaire</term><term>Gènes de plante</term><term>Interférence par ARN</term><term>Régulation de l'expression des gènes végétaux</term><term>Techniques de knock-down de gènes</term><term>Transduction génétique</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The exploding availability of genome and EST-based sequences from grasses requires a technology that allows rapid functional analysis of the multitude of genes that these resources provide. There are several techniques available to determine a gene's function. For gene knockdown studies, silencing through RNAi is a powerful tool. Gene silencing can be accomplished through stable transformation or transient expression of a fragment of a target gene sequence. Stable transformation in rice, maize, and a few other species, although routine, remains a relatively low-throughput process. Transformation in other grass species is difficult and labor-intensive. Therefore, transient gene silencing methods including Agrobacterium-mediated and virus-induced gene silencing (VIGS) have great potential for researchers studying gene function in grasses. VIGS in grasses already has been used to determine the function of genes during pathogen challenge and plant development. It also can be used in moderate-throughput reverse genetics screens to determine gene function. However, the number of viruses modified to serve as silencing vectors in grasses is limited, and the silencing phenotype induced by these vectors is not optimal: the phenotype being transient and with moderate penetration throughout the tissue. Here, we review the most recent information available for VIGS in grasses and summarize the strengths and weaknesses in current virus-grass host systems. We describe ways to improve current virus vectors and the potential of other grass-infecting viruses for VIGS studies. This work is necessary because VIGS for the foreseeable future remains a higher throughput and more rapid system to evaluate gene function than stable transformation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23386292</PMID><DateCompleted><Year>2013</Year><Month>07</Month><Day>15</Day></DateCompleted><DateRevised><Year>2013</Year><Month>02</Month><Day>06</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>975</Volume><PubDate><Year>2013</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Rationale for developing new virus vectors to analyze gene function in grasses through virus-induced gene silencing.</ArticleTitle><Pagination><MedlinePgn>15-32</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-62703-278-0_2</ELocationID><Abstract><AbstractText>The exploding availability of genome and EST-based sequences from grasses requires a technology that allows rapid functional analysis of the multitude of genes that these resources provide. There are several techniques available to determine a gene's function. For gene knockdown studies, silencing through RNAi is a powerful tool. Gene silencing can be accomplished through stable transformation or transient expression of a fragment of a target gene sequence. Stable transformation in rice, maize, and a few other species, although routine, remains a relatively low-throughput process. Transformation in other grass species is difficult and labor-intensive. Therefore, transient gene silencing methods including Agrobacterium-mediated and virus-induced gene silencing (VIGS) have great potential for researchers studying gene function in grasses. VIGS in grasses already has been used to determine the function of genes during pathogen challenge and plant development. It also can be used in moderate-throughput reverse genetics screens to determine gene function. However, the number of viruses modified to serve as silencing vectors in grasses is limited, and the silencing phenotype induced by these vectors is not optimal: the phenotype being transient and with moderate penetration throughout the tissue. Here, we review the most recent information available for VIGS in grasses and summarize the strengths and weaknesses in current virus-grass host systems. We describe ways to improve current virus vectors and the potential of other grass-infecting viruses for VIGS studies. This work is necessary because VIGS for the foreseeable future remains a higher throughput and more rapid system to evaluate gene function than stable transformation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ramanna</LastName><ForeName>Hema</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Plant Biology Division, The Samuel Roberts Noble Foundation Inc., Ardmore, OK, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Xin Shun</ForeName><Initials>XS</Initials></Author><Author ValidYN="Y"><LastName>Nelson</LastName><ForeName>Richard S</ForeName><Initials>RS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D016454">Review</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019177" MajorTopicYN="N">Bromoviridae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055785" MajorTopicYN="N">Gene Knockdown Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058977" MajorTopicYN="N">Molecular Sequence Annotation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009029" MajorTopicYN="N">Mosaic Viruses</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006109" MajorTopicYN="N">Poaceae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="Y">RNA Interference</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014161" MajorTopicYN="N">Transduction, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>2</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>2</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23386292</ArticleId><ArticleId IdType="doi">10.1007/978-1-62703-278-0_2</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Oklahoma</li></region></list><tree><noCountry><name sortKey="Ding, Xin Shun" sort="Ding, Xin Shun" uniqKey="Ding X" first="Xin Shun" last="Ding">Xin Shun Ding</name><name sortKey="Nelson, Richard S" sort="Nelson, Richard S" uniqKey="Nelson R" first="Richard S" last="Nelson">Richard S. Nelson</name></noCountry><country name="États-Unis"><region name="Oklahoma"><name sortKey="Ramanna, Hema" sort="Ramanna, Hema" uniqKey="Ramanna H" first="Hema" last="Ramanna">Hema Ramanna</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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